PCR Equipment and Supplies
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Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | High |
| Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | High-Fidelity PCR Kit |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Tissue |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Label or Dye | VIC |
| Species | Not Species-Specific |
| Product Line | VetMAX, Xeno |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | 1-Step qPCR kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | Cells-to-CT, TaqMan |
| Detection Method | TaqMan |
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 5 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
Thermo Scientific™ dNTP Mix (25 mM each)
Thermo Scientific dNTP Mix (25 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | >99% |
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 25 mM |
| For Use With (Application) | Standard PCR,High-fidelity PCR,RT-PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)
Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Product Type | DNA Polymerase |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Exonuclease Activity | 5' - 3' |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| Format | Kit |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ VetMAX™ PRRSV 3.0 Reagents
VetMAX™ PRRSV 3.0 Reagents contain master mix, Xeno RNA control, and a primers/probes mix that enables multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) assays targeting North American (NA) and European (EU) porcine reproductive and respiratory syndrome virus (PRSSV) RNA and Xeno RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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