PCR Equipment and Supplies
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Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Label or Dye | VIC |
| Species | Not Species-Specific |
| Product Line | VetMAX, Xeno |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Taq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Format | Separate Components |
| Product Type | Taq DNA Polymerase (Recombinant) |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | Hot Start DNA Polymerase |
| Reaction Speed | Fast or Standard |
| Overhang | 3'-A |
| Concentration | 2X |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 8 kb |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Universal Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | ArrayScript™ UP |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Run Time | Standard |
| Product Line | RNA-to-CT, SYBR |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| Format | Kit |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
| Concentration | 200 U/μL |
|---|
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |